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CS-HAEO-Ne depicted excellent in situ efficacy by inhibiting fungal infestation, AFB 1 contamination, lipid peroxidation, and mineral loss with acceptable sensorial profile. CS-HAEO-Ne exhibited superior antioxidant efficacy (IC 50 (DPPH) = 4.5 μL/mL) over unencapsulated HAEO (IC 50 (DPPH) = 15.9 μL/mL) and phenolic content. flavus cells illustrated the novel antiaflatoxigenic efficacy of CS-HAEO-Ne. Depletion of methylglyoxal level in treated A. flavus and provoked leakage of cellular ions (Ca +2, Mg +2, and K +) as well as 260 nm and 280 nm absorbing materials. CS-HAEO-Ne caused significant reduction in ergosterol content in treated A. flavus growth and AFB 1 biosynthesis at 1.25 μL/mL and 1.0 μL/mL, respectively in comparison to unencapsulated HAEO (1.75 μL/mL and 1.25 μL/mL, respectively). HAEO was encapsulated into chitosan nanomatrix (CS-HAEO-Ne) and characterized through SEM, FTIR, and XRD. GC–MS analysis revealed linalool (68.51%) as the major component of HAEO. It is a complete offline setup of RA Beauty Retouch Panel for Photoshop 2021 for macOS with a single click download link.Present study deals with the efficacy of nanoencapsulated Homalomena aromatica essential oil (HAEO) as a potent green preservative against toxigenic Aspergillus flavus strain (AF-LHP-NS 7), storage fungi, AFB 1, and free radical-mediated deterioration of stored spices.
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